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Columns for preparative chromatography

Separchrom PC01



High performance Columns with biaxial compression

Columns Separchrom PC01 correspond to the highest requirements of preparative liquid chromatography. PC01 columns are designed to be used in a mode of axial (or biaxial) compression. Columns can be packed by dynamic slurry technique or by sedimentation method. They are equipped with identical pistons on input and output. Pistons are provided by Poremet® 2 stainless steel (AISI 316 L) frits with porosity 3 µm and minimum hydraulic resistance. New developed patented liquid distributing system is installed under each frit. The design guarantees perfect piston flow, high efficiency even for high injection volume and simple accessibility of frit for cleaning.

Separchrom PC01 columns are made of high quality stainless steel (AISI 316 or AISI 316L on the request) and their inner surface is polished to reach Ra < 0,3 µm. Column flanges are not welded, but screwed on the column tube. It means that there is no temperature increasing during manufacture and thus no change of steel properties. Columns have input piston with large stroke (possibly through whole column length) and it is moved by a force generated by a hydraulic cylinder or by flange bolts tightening. Single hydraulic cylinders fitted on upper flange with manual oil pumps are offered as well as fully automated electric motor driven devices for columns’ packing/unpacking procedure. Separchrom PC01 column output pistons movement is provided mechanically by tightening the flange bolts. In this case is functional stroke of the piston approximately 15 % of the column I.D. Pistons are sectional, frit squeezed to the frit ring can be released from piston body and cleaned as well the flow distributor. Distributor design effectively protects the frit from particles which can caused i´their blockage.

Separchrom PC01 columns are delivered with legs made of stainless steel closed profiles and fitted to the upper tube flange. It means that output flange is fully accessible for disassembling and sorbent releasing.

Separchrom PC01 columns are completed by a broad scale of accessories as filling adapters, slurry mixing vessels, slurry transporting pumps etc. for automatic filling using dynamic slurry technique as well as automatic emptying procedure.

Separchrom PC02



Columns for Medium Pressure with axial bed compression

Separchrom PC02 medium pressure stainless steel column are intended for separation where high pressure is not necessary, but high efficiency can be still expected and useful. Separchrom PC02 thus fill requirements of many users. They are made of cutted and polished stainless steel tubes and equipped on input either with ultra high molecular weight polyethylene made pistons (PC 02 UP) with steel support or stainless steel made pistons (PC 02 SP), both with Poremet® 5 stainless steel (AISI 316 L) frits with 02 porosity 5 µm and minimum hydraulic resistance. Flow distributors with groves for liquid distribution are incorporated in to the piston and the system guarantees perfect piston flow with nearly zero pressure drop. Columns have functional stroke of the input piston approximately 60 % of the inner diameter of the column when working with flange bolts and even higher when hydraulic cylinder is used.

Outputs of PC02 columns are designed as fix units (pistons on special request) and use the same flow distributors and frits like input pistons.

Due the low movement resistance of UHMWE pistons Separchrom PC02 columns can be equipped with special software application in order to compensate swelling and deswelling of organic sorbents in different mobile phases (column piston is moved in very small steps up and down during use).

Separchrom PC04



Columns for Medium Pressure with axial bed compression

For flash chromatography are usually intended polyethylene cartridge columns. Metal free columns are requested in some special applications too. Separlab offers Separchrom PC04 columns for both these purposes. Columns are made of high quality ultra high molecular weight polyethylene, have similar design

like stainless steel columns PC01 or PC02 and are equipped with polypropylene fabrics (non woven polypropylene 10 µm pores) made frits. Piston on the input moved by a robust central screw increases the efficiency due an elimination of free space between upper sorbent layer and frit with low pressure flow distributor. Output frit with flow distributor is incorporated in output flange. Columns are compatible with nearly all organic solvents and with all water solutions. Columns are designated for low pressure and medium pressure liquid chromatography.

Separchrom PC06

pc 06


Columns for Medium Pressure without bed compression

Separchrom PC06 are intended for applications where sorbent does not change the volume during the use. Columns are equipped with frits and distributors incorporated in to input and output flanges. They are made of polished stainless steel tubes and equipped with non woven polypropylene frits with porosity 10 µm. Flow distributors are incorporated in to column flanges.

Separchrom PC08



SEPARCHROM PC08 columns are designed for high pressure, high performance preparative liquid chromatography. They are equipped by pistons on both ends. All metal parts in connection with mobile phase are made of AISI 316L stainless steel. Upper piston movement is secured by hydraulics, but in comparison with standard models, PC08 hydraulics is in incorporated into bottom column stand, own column is fixed in a robust frame and hydraulic piston press the whole column unit against a special flange in most upper part of the system.

PC08 columns are used for high performance separations in instances where small rigid particles are used as column filling. Only stainless steel and UHMWPE (ultra high molecular weight polyethylene) are in contact with mobile phase. Columns are resisting to all common solvents. PC08 columns with are designed for industrial separations and typically are working with flow rate 400 ml/min. – 1400 ml/min. depending on sorbent type and separation mode. Maximal column pressure is 120 bar.

PC08 columns are designed either for sedimentation packing with following axial compression of sorbent bed or for dynamic axial compression packing. Columns with I.D. 150 mm or 200 mm have a special stand with motor driven high pressure hydraulic aggregate and hydraulic cylinder (40 tons, 200 - 600 mm stroke, double action) which serves for sorbent pressing during the use to compensate bed volume changes as well.

PC08 column stand has hydraulic system in bottom part and column is fixed between hydraulic cylinder piston and upper stand flange which is connected to the stand box by five rods. Column itself can be removed from the frame without deassembling of the frame

Typically the PC08 column has the same design as PC 01 columns - consists of tube, I.D. 150 mm or 200 mm with the length 400 - 700 mm. The internal surface of the column is mechanically polished to attain high smoothness. The tube is provided with two screwed stainless steel flanges (tube flanges). Column pistons are equipped with Poremet® 2 frits (3 um porosity) and flow distributors.




Hydraulic system SEPARPRESS for prep chromatographic columns. Used to compress the piston inside the column and to compact the sorbent. Supplied as a kit that includes a hydraulic cylinder - double action (D) or with spring (S) with connecting hoses, oil pump - manual (M) or driven by motor (E) with control device - mechanical manometer (M) or electronic system with pressure gauge (E).